Coding

Part:BBa_J119056:Design

Designed by: Michel Conn   Group: Eckdahl Lab   (2012-01-26)

pLacI-RBS-T7 RNAP (2SAT mutant 2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 148
    Illegal BsaI.rc site found at 99


Design Notes

The frameshift mutation is of the form (b’ or c’)and(a or b).



Source

Existing part and synthetic oligos.

References